TR FRET Assay Kit

KeyTec® TR-FRET Human CD64 (FcγRI) Binding Kit is designed to screen IgGs that binds to Human CD64 (FcγRI), and provides an ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD64 (FcγRI) is hereinafter referred as CD64. This kit is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

The KeyTec^® TR‑FRET CDKs Kinase Kit quantifies CDK activity via phosphorylation of Rb S780. It includes a LA‑labeled substrate and a Solar Eu‑labeled phospho‑antibody. The TR‑FRET sandwich assay provides simple, rapid, and sensitive quantification.

KeyTec® TR-FRET BRD4 / VHL PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between BRD4 protein and the VHL/Elongin C/Elongin B protein complex (VHL/Elongin C/Elongin B protein complex, hereinafter referred to as the VCB complex).

KeyTec® TR-FRET mAb anti-GST-HX is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-HX*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

The KeyTec^® TR‑FRET CDKs Kinase Kit detects activity of CDK2 and CDK5, which phosphorylate ATF‑2 at T71. It supplies a LA‑labeled substrate and a Solar Eu‑labeled phospho‑antibody. The TR‑FRET sandwich assay involves kinase reaction (substrate phosphorylation) and detection (antibody binding to phosphorylated site), generating a signal proportional to activity. The assay is simple, rapid, sensitive, and reproducible.

This kit provides a homogeneous competitive TR-FRET assay for screening inhibitors of the TL1A–DR3 protein–protein interaction. Using a donor-labeled Streptavidin/TL1A complex and an acceptor-labeled DR3, the assay detects the binding signal at 665 nm. Compounds that inhibit the interaction reduce the signal in a concentration-dependent manner, enabling rapid and reliable screening of TL1A/DR3 blockers.

The KeyTec® TR‑FRET Human SMARCA2 Protein Binding Assay Kit is a competitive immunoassay for screening ligands and inhibitors of wild‑type SMARCA2.  The assay is rapid, sensitive, and reproducible, making it suitable for high‑throughput screening applications.

KeyTec® TR-FRET TKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.

This kit offers a homogeneous competitive TR-FRET assay for screening inhibitors of the TL1A–DcR3 protein–protein interaction. Using a donor-labeled anti-Tag1 antibody to recognize Tag1-TL1A and an acceptor-labeled DcR3, the assay detects binding signals at 665 nm. Inhibitors that block the interaction reduce the signal in a concentration-dependent manner, enabling rapid and reliable screening of TL1A/DcR3 blockers.

The KeyTec® TR‑FRET Human PD1/PD‑L2 Binding Assay Kit provides a rapid, accurate, and homogeneous method for high‑throughput screening of blockers of the PD1–PD‑L2 interaction. This format is ideal for evaluating small molecules, peptides, and antibodies.

KeyTec^® TR-FRET BTKs substrate - biotin is designed for measuring the Tyrosine kinase (TKs) activity with KeyTec® TR-FRET TKs kinase kit.

This kit provides a homogeneous sandwich TR-FRET assay for rapid screening and affinity ranking of mouse hybridoma antibodies in culture supernatants. Using a donor-labeled anti-FLAG antibody to capture the FLAG-tagged antigen and an acceptor-labeled anti-mouse IgG antibody to detect the hybridoma-derived antibody, the assay measures proximity-dependent energy transfer signals at 665 nm, allowing quick and reliable identification of high-affinity positive clones.

The KeyTec® TR‑FRET Human SMARCA4 Protein Binding Assay Kit is a competitive, homogeneous immunoassay for screening ligands and inhibitors of wild‑type SMARCA4. It measures the interaction between SMARCA4 and a bromodomain ligand via TR‑FRET, with signal decreasing as competitor concentration increases. 

This kit provides a rapid, homogeneous TR-FRET assay for screening PROTACs that promote ternary complex formation between BRD4 and CRBN/DDB1. Using two labeled tag-specific antibodies, the assay measures the proximity-induced energy transfer signal at 665 nm, enabling reliable and quantitative evaluation of PROTAC-mediated protein-protein interactions.

This kit provides a homogeneous TR-FRET assay for high-throughput screening of PROTACs that induce ternary complex formation between ALK and CRBN/DDB1. Using two tag-specific antibodies labeled with donor and acceptor fluorophores, the assay measures proximity-dependent energy transfer signals at 665 nm, offering a rapid, reliable, and quantitative approach for evaluating PROTAC-mediated protein–protein interactions.

This kit offers a homogeneous TR-FRET assay for screening PROTACs that promote ternary complex assembly between NEK7 and CRBN/DDB1. Utilizing donor-labeled anti-Tag1 and acceptor-labeled anti-Tag2 antibodies, the assay detects proximity-induced energy transfer at 665 nm, providing a rapid, accurate, and reproducible method for quantitative evaluation of PROTAC-mediated NEK7–CRBN/DDB1 interactions.