VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
The KeyTec® TR‑FRET FGFRs substrate‑biotin kinase substrate can be used to develop kinase activity assays. When combined with the KeyTec® TR‑FRET TKs kinase kit (Cat. # A1080001L), it enables the construction of a TR‑FRET activity assay for evaluating relative enzyme activity or inhibitor screening.
The KeyTec® ActRIIA Kinase Kit detects ActRIIA activity by quantifying ADP produced from substrate phosphorylation. The assay includes a kinase reaction and an ADP detection step, enabling rapid kinase activity assessment
KeyTec® TR-FRET mAb anti-FLAG-Solar Tb is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-FLAG-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Human CD16a (FcγRIIIA, 176F) Binding Kit is designed to screen IgGs that bind to Human CD16a (FcγRⅢA, 176F), and provides the ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD16a (FcγRIIIA, 176F) is hereinafter referred as CD16a (176F). This assay is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
The KeyTec® ActRIIB Kinase Kit detects relative ActRIIB (ACVR2B) activity by quantifying ADP generated from a kinase–substrate reaction.
KeyTec® TR-FRET mAb anti-FLAG-LA is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Human CD64 (FcγRI) Binding Kit is designed to screen IgGs that binds to Human CD64 (FcγRI), and provides an ideal solution to assess the ADCC and ADCP of antibody candidate drugs. Human CD64 (FcγRI) is hereinafter referred as CD64. This kit is based on a competitive immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
KeyTec® TR-FRET mAb anti-FLAG-HX is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG- HX*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-MYC-Solar Eu is designed for developing the TR-FRET Assay. The anti-MYC antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one MYC-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-MYC-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-MYC-LA is designed for developing the TR-FRET Assay. The anti-MYC antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one MYC-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-MYC- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-HA-Solar Eu is designed for developing the TR-FRET Assay. The anti-HA antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HA-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HA-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-HA-LA is designed for developing the TR-FRET Assay. The anti-HA antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HA-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HA- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-Solar Eu is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
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