VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET mAb anti-MYC-LA is designed for developing the TR-FRET Assay. The anti-MYC antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one MYC-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-MYC- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-HA-Solar Eu is designed for developing the TR-FRET Assay. The anti-HA antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HA-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HA-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-HA-LA is designed for developing the TR-FRET Assay. The anti-HA antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HA-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HA- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-Solar Eu is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-Solar Tb is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the donor (KeyTec® TR-FRET Streptavidin-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-LA is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the acceptor (KeyTec® TR-FRET Streptavidin- LA*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET Streptavidin-HX is designed for developing the TR-FRET Assay. In the Protein-Protein Interaction assay, one Biotinylated protein binds to the acceptor (KeyTec® TR-FRET Streptavidin-HX*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET pAb anti-Human IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Human IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Human IgG-LA is designed for developing the TR-FRET Assay. The anti- Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Human IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Human IgG-HX is designed for developing the TR-FRET Assay. The anti- Human IgG antibody is a mouse polyclonal antibody. In the Antigen-Antibody Interaction assay, the Human IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Human IgG-HX*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Mouse IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Mouse IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Mouse IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Mouse IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Mouse IgG-LA is designed for developing the TR-FRET Assay. The anti-Mouse IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Mouse IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Mouse IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rabbit IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Rabbit IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rabbit IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Rabbit IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rabbit IgG-LA is designed for developing the TR-FRET Assay. The anti-Rabbit IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rabbit IgG antibody binds to the acceptor (KeyTec® TR-FRET pAb anti-Rabbit IgG-LA*1), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu/Tb*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET pAb anti-Rat IgG-Solar Eu is designed for developing the TR-FRET Assay. The anti-Rat IgG antibody is a goat polyclonal antibody. In the Antigen-Antibody Interaction assay, the Rat IgG antibody binds to the donor (KeyTec® TR-FRET pAb anti-Rat IgG-Solar Eu*1), and the antigen is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
English
