TR FRET Assay Kit

KeyTec® TR-FRET mAb anti-HIS-Solar Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

The KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.

The KeyTec® TR-FRET GDP loaded KRAS[G12D]/SOS1 Binding Assay kit is designed for measurement of the interaction between KRAS[G12D] and SOS1 proteins. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, high accuracy.

The KeyTec^® TR‑FRET Revamped TKs Kinase Assay Kit quantifies tyrosine kinase activity using a universal substrate or customized tyrosine‑specific substrates, compatible with >60 TKs. It employs a biotinylated peptide and streptavidin‑HX, along with a Solar Eu‑labeled anti‑phospho‑Tyr antibody, in a TR‑FRET sandwich format. The assay involves kinase reaction and detection steps, producing a signal proportional to phosphorylation. Simple, rapid, sensitive, and reproducible.

KeyTec^® cAMP TR-FRET kit can be used to measure cAMP levels in cells. This kit is a competitive immunoassay developed using KeyTec^® TR-FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility.

KeyTec^® TR-FRET Human IL2 Detection Kit is designed for the quantitative determination of Human IL2 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec^® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec^® TR-FRET Human IL1β Detection Kit is designed for the quantitative determination of Human IL1β in supernatants. This assay is based on a sandwich immunoassay method using KeyTec^® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

KeyTec^® TR-FRET Human IFNγ Detection Kit is designed for the quantitative determination of Human IFNγ in supernatants. This assay is based on a sandwich immunoassay method using KeyTec^® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.

This kit utilizes KeyTec® TR-FRET technology for high‑throughput screening, enabling accurate measurement of IL17F/IL17RA protein‑protein interactions and assessment of the activities of small‑molecule inhibitors, peptides, and antibody‑blocking agents.

This kit utilizes KeyTec® TR-FRET technology for high‑throughput screening, enabling accurate measurement of IL17A/IL17RA protein‑protein interactions and assessment of the activities of small‑molecule inhibitors, peptides, and antibody‑blocking agents.

The KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit is designed for measurement of the interaction between KRAS[G12D] and RAF1 proteins. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, high accuracy.

KeyTec^® TR-FRET cAMP HiRange Detection kit can be used to measure cAMP levels in cells. This kit is a competitive immunoassay developed using KeyTec^® TR-FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility.

KeyTec® TR-FRET mAb anti-HIS-Solar Tb is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520 nm, proportional to the extent of protein interaction.

KeyTec^® TR-FRET PD1/PD-L1 Binding Assay Kit is designed for the quantitative measurement of the interaction between human PD1 and PD-L1 proteins. This kit provides a Tag1-PD-L1 protein, a Tag2-PD1 protein, an anti-tag1 antibody labeled with KeyTec^® TR-FRET Solar Eu* ¹ (mAb anti-Tag1 - Solar Eu) ,an anti-tag2 antibody labeled with KeyTec^® TR-FRET LA* ² (mAb anti-Tag2 - LA) and other detection reagents. These components enable the high-throughput screening of small molecule inhibitors, peptides, or antibody blockers.

KeyTec^® TR-FRET cAMP Higher Detection kit can be used to measure cAMP levels in cells. This kit is a competitive immunoassay developed using KeyTec^® TR-FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility.

This kit measures serine/threonine kinase (STK) activity, validated for over 150 STKs. It supplies three biotinylated substrates (S1/S2/S3), streptavidin‑HX, and a phospho‑STK antibody labeled with Solar Eu, enabling quantification via phosphorylated substrate detection.