VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET STK discovery kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 150 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides three types of biotinylated substrates(S1/S2/S3), Streptavidin-HX* 1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu* 2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrates.
KeyTec® TR-FRET STK S1 kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 50 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
KeyTec® TR-FRET STK S2 kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 30 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
KeyTec® TR-FRET STK S3 kinase kit is designed for measuring the Serine/threonine kinase (STKs) activity. Serine/threonine kinase family comprises multiple members. This assay kit has been validated for activity measurement of over 50 Serine/threonine kinases (For more details, please visit our official website or contact our technical support team). The assay provides a biotinylated substrate, Streptavidin-HX*1 , a phospho STK-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
KeyTec® TR-FRET mAb anti-HIS-Solar Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET CDKs kinase kit is designed for measuring the cyclin-dependent kinases (CDKs) activity. The protien kinase family, which comprises multiple members such as CDK1, 3, 4, 6, 7, 9, 10, 12, 13, 15, 16, 17 and 18, is known to phosphorylate serine 780 (S780) of the retinoblastoma-associated protein (Rb). The assay kit provides a CDKs-specific substrate labeled with KeyTec® TR-FRET LA*1 , a phospho-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
KeyTec® TR-FRET CDKs kinase detection kit is designed for measuring the cyclin-dependent kinases (CDKs) activity. The protien kinase family , which comprises multiple members such as CDK2 and 5, is known to phosphorylate Threonine 71 (T71) of Activating transcription factor 2 (ATF-2). The assay kit provides a CDKs-specific substrate labeled with KeyTec® TR-FRET LA*1 , a phospho-specific antibody labeled with KeyTec® TR-FRET Solar Eu*2 and detection reagent. These components enable the quantification of kinase activity by detecting the phosphorylated substrate.
KeyTec® TR-FRET mAb anti-HIS-Solar Tb is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520 nm, proportional to the extent of protein interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
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