VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.
KeyTec® TR-FRET mAb anti-HIS-Solar Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Eu*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
The KeyTec® TR-FRET GDP loaded KRAS[G12D]/SOS1 Binding Assay kit is designed for measurement of the interaction between KRAS[G12D] and SOS1 proteins. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, high accuracy.
The KeyTec® TR‑FRET Revamped TKs Kinase Assay Kit quantifies tyrosine kinase activity using a universal substrate or customized tyrosine‑specific substrates, compatible with >60 TKs. It employs a biotinylated peptide and streptavidin‑HX, along with a Solar Eu‑labeled anti‑phospho‑Tyr antibody, in a TR‑FRET sandwich format. The assay involves kinase reaction and detection steps, producing a signal proportional to phosphorylation. Simple, rapid, sensitive, and reproducible.
KeyTec® cAMP TR-FRET kit can be used to measure cAMP levels in cells. This kit is a competitive immunoassay developed using KeyTec® TR-FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility.
KeyTec® TR-FRET Human IL2 Detection Kit is designed for the quantitative determination of Human IL2 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
KeyTec® TR-FRET Human IL1β Detection Kit is designed for the quantitative determination of Human IL1β in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
KeyTec® TR-FRET Human IFNγ Detection Kit is designed for the quantitative determination of Human IFNγ in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
This kit utilizes KeyTec® TR-FRET technology for high‑throughput screening, enabling accurate measurement of IL17FF/IL17RA protein‑protein interactions and assessment of the activities of small‑molecule inhibitors, peptides, and antibody‑blocking agents.
The KeyTec® TR-FRET AKT (S473) Phosphorylation Assay Kit is a sandwich immunoassay that rapidly and specifically quantifies AKT S473 phosphorylation in cell lysates using TR-FRET technology. It features high sensitivity, good reproducibility, and a simple workflow, with signal readout at 665 nm reflecting the phosphorylation level.
KeyTec® TR-FRET Human IL4 Detection Kit is designed for the quantitative determination of Human IL4 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.
The KeyTec® TR-FRET Human Fc detection kit is designed for quantitative measurement of human IgG or human Fc-tagged protein in supernatant. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
KeyTec® TR-FRET cAMP HiRange Detection kit can be used to measure cAMP levels in cells. This kit is a competitive immunoassay developed using KeyTec® TR-FRET technology, featuring simple and rapid operation, high specificity, high sensitivity, and excellent reproducibility.
This kit utilizes KeyTec® TR-FRET technology for high‑throughput screening, enabling accurate measurement of IL17AA/IL17RA protein‑protein interactions and assessment of the activities of small‑molecule inhibitors, peptides, and antibody‑blocking agents.
KeyTec® TR-FRET mAb anti-HIS-Solar Tb is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Tb*1), and the other protein is labeled (directly or indirectly) with the acceptor (KeyTec® TR-FRET LA/HX/Green*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm or 520 nm, proportional to the extent of protein interaction.
Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.
TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.
The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.
Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.
VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.
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