TR FRET Assay Kit

KeyTec® TR-FRET mAb anti-FLAG-Green is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.

This Green2-labeled anti-HA tag antibody serves as an acceptor reagent for TR-FRET-based protein-protein interaction assays. When paired with Solar Tb1-labeled anti-His tag antibody, it enables detection of interactions between His-tagged and HA-tagged proteins. The proximity-dependent FRET signal, read at 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.

This Green2-labeled Streptavidin serves as an acceptor reagent for TR-FRET-based protein-protein interaction assays. When paired with Solar Tb1-labeled anti-His tag antibody, it enables detection of interactions between His-tagged proteins and biotinylated binding partners. The proximity-dependent FRET signal, read at 520 nm, correlates with binding strength, providing a homogeneous and high-throughput platform for screening protein-protein interaction modulators.

KeyTec® TR-FRET Bright Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the Donor (KeyTec® TR-FRET mAb anti-HIS-Bright Eu*1), and the other protein is labeled (directly or indirectly) with the Acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

This TR-FRET-based immunoassay uses Eu1-labeled anti-GST and LA2-labeled anti-His antibodies to detect proximity between GST- and His-tagged interacting proteins. Upon donor excitation, FRET produces a 665‑nm signal proportional to binding strength, enabling robust screening of PPI modulators such as small-molecule inhibitors or blocking antibodies.

This TR-FRET-based assay employs an Eu1-labeled anti-FLAG antibody and an LA2-labeled anti-His antibody to monitor the proximity of FLAG- and His-tagged interacting proteins. Upon donor excitation, FRET generates a 665‑nm emission signal that correlates directly with binding strength, providing a reliable platform for high-throughput screening of PPI modulators, including small-molecule inhibitors and neutralizing antibodies.

This Bright Tb1-labeled Streptavidin serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled anti-His tag antibody, it enables detection of interactions between biotinylated proteins and His-tagged partners. The proximity-dependent FRET signal, readable at both 665 nm and 520 nm, correlates with binding strength, offering a versatile and high-throughput platform for screening protein-protein interaction modulators.

This Bright Tb1-labeled anti-His tag antibody serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled Streptavidin, it enables detection of interactions between His-tagged proteins and biotinylated binding partners. The proximity-dependent FRET signal, measurable at both 665 nm and 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.

This Bright Tb1-labeled anti-GST tag antibody serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled anti-His tag antibody, it enables detection of interactions between GST-tagged and His-tagged proteins. The proximity-dependent FRET signal, measurable at both 665 nm and 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.

This Bright Tb1-labeled anti-FLAG tag antibody serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled anti-His tag antibody, it enables detection of interactions between FLAG-tagged and His-tagged proteins. The proximity-dependent FRET signal, measurable at both 665 nm and 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.

This TR-FRET-based assay utilizes a Tb1-labeled anti-HA antibody and an Acceptor2-labeled anti-His antibody to detect proximity between HA- and His-tagged interacting proteins. Upon donor excitation, FRET produces emission signals at both 665 nm and 520 nm, with signal intensities reflecting the binding strength. This dual-wavelength readout offers a robust and flexible platform for high-throughput screening of PPI modulators, including small-molecule inhibitors and blocking antibodies.