Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
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TR FRET Assay Kit

VKEY-BIO's TR-FRET assay kits utilize advanced time resolved fluorescence resonance energy transfer (TR-FRET) technology, delivering rapid, wash-free operation with exceptional sensitivity, low background, and outstanding stability. These ready-to-use kits are optimized for diverse applications including protein-protein interaction studies, biomarker detection (cytokines/cAMP), phosphoprotein analysis, hybridoma screening, and antibody quantification.

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KeyTec® TR-FRET Human IL8 Detection kit
KeyTec® TR-FRET Human IL8 Detection kit

KeyTec® TR-FRET Human IL8 Detection Kit is designed for the quantitative determination of Human IL8 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.


CAT. / A1070004S / A1070004L
KeyTec® TR-FRET Human IL6 Detection kit
KeyTec® TR-FRET Human IL6 Detection kit

KeyTec® TR-FRET Human IL6 Detection Kit is designed for the quantitative determination of Human IL6 in supernatants. This assay is based on a sandwich immunoassay method using KeyTec® TR-FRET technology, offering a simple, rapid, highly specific and sensitive, as well as reproducible detection process.


CAT. / A1070003S / A1070003L
KeyTec® TR-FRET Human TNFα Detection kit
KeyTec® TR-FRET Human TNFα Detection kit
The KeyTec® TR-FRET Human TNFαassay kit is designed for quantitative measurement of Human TNFα protein in supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
CAT. / A1070001S / A1070001L
KeyTec® TR-FRET cAMP HiRange Detection kit
KeyTec® TR-FRET cAMP HiRange Detection kit
KeyTec® TR-FRET cAMP HiRange Kit enables sensitive, high-throughput cAMP detection for GPCR studies. Fast, reliable, and optimized for cell-based assays.
CAT. / A1030003S / A1030003L / A1030003B
KeyTec® TR-FRET Binding Assay Diluent Buffer
KeyTec® TR-FRET Binding Assay Diluent Buffer
CAT. / A1010001S / A1010001L / A1010001B
KeyTec® TR-FRET Solar Eu Detection Buffer
KeyTec® TR-FRET Solar Eu Detection Buffer
CAT. / A1010002S / A1010002L / A1010002B
KeyTec® TR-FRET Cytokine Diluent Buffer
KeyTec® TR-FRET Cytokine Diluent Buffer
CAT. / A1010006L / A1010006S
KeyTec® TR-FRET Kinase Enzymatic buffer
KeyTec® TR-FRET Kinase Enzymatic buffer
CAT. / A1010018B / A1010018L / A1010018S
KeyTec® TR-FRET Kinase Detection buffer
KeyTec® TR-FRET Kinase Detection buffer
CAT. / A1010019B / A1010019L / A1010019S
KeyTec® TR-FRET Kinase Binding buffer
KeyTec® TR-FRET Kinase Binding buffer
CAT. / A1010020L / A1010020S
KeyTec® TR-FRET PROTAC Diluent Buffer
KeyTec® TR-FRET PROTAC Diluent Buffer
CAT. / A1010025S / A1010025L
TR-FRET Assay Working Principle

TR-FRET Assay Working Principle

Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology combines the advantages of Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). Based on the TR-FRET principle, this method utilizes the long fluorescence lifetime and high stability of lanthanide elements to deliver superior performance. Our TR-FRET assay kits provide an easy-to-use, homogeneous experimental approach that eliminates the need for washing. The Time Resolved Fluorescence Energy Transfer technology ensures enhanced stability, lower background, improved signal-to-noise ratio, and greater sensitivity, making it an ideal solution for robust detection.

Advantages of VKEY-BIO's TR-FRET Assay Kits

Easy to Use
Easy to Use

TR-FRET assay kits eliminate complex wash steps, simplifying protocol while delivering lab-ready consistency.

Good Specificity and High Sensitivity
Good Specificity and High Sensitivity

The TR-FRET assay kits deliver exceptional specificity and sensitivity, enabling reliable detection of low-abundance targets.

Suitable for High Throughput Screening
Suitable for High Throughput Screening

Designed for efficiency, our TR-FRET assay kits are optimized for high-throughput screening in 384-well formats.

Good Stability and Repeatability
Good Stability and Repeatability

VKEY-BIO's TR-FRET assay kits demonstrate outstanding batch-to-batch stability and experimental repeatability for consistent results.