In monoclonal antibody development, hybridoma screening is a critical bottleneck. Traditional methods (like ELISA) are time-consuming (often requiring overnight incubation), involve cumbersome steps, have limited throughput, and are susceptible to matrix interference. Facing thousands of hybridoma clones, researchers urgently need a rapid, sensitive, and easy-to-operate high-throughput screening tool.
KeyTec® TR-FRET Hybridoma Screening Kit ------ Based on Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology, it enables accurate assessment of antibody affinity within 2 hours, pressing the "fast-forward button" for mAb development.
This series of kits offers a free combination scheme of "Antigen Tag + Antibody Species," comprehensively covering mainstream research needs:
Dual Probe System:
Anti-His Antibody conjugated to TR-FRET donor (Solar Eu) → Binds His-tagged antigen
Anti-Mouse Fc Antibody conjugated to TR-FRET acceptor (LA) → Binds mouse IgG antibody
"Sandwich" structure formation: Antigen-antibody binding brings the donor and acceptor into proximity, enabling energy transfer upon excitation.
Signal detection: The 665 nm / 615 nm fluorescence ratio (TR-FRET Ratio) is read to quantify antibody affinity.
KeyTec® TR-FRET Hybridoma Antibody Screening Kit principle
Biotin Interference Solution: The biotinylated antigen kit features a unique pre-incubation design (Streptavidin-Solar Eu + Biotinylated antigen), effectively eliminating interference from free biotin in the culture medium.
Ready-to-Use Buffer: The kit provides an optimized Hybridoma Detection Buffer, ensuring result stability.
Lyophilized Positive Control: Easy reconstitution reduces batch-to-batch variation.
Basic Research: Rapidly screen hundreds of hybridoma clones to accelerate antibody discovery.
Therapeutic Antibody Development: Screen high-affinity clones for targets like PD-L1, TNFα, etc.
Diagnostic Reagent R&D: Ensure antibody sensitivity and specificity, reducing development risks.
Vaccine Evaluation: Screen neutralizing antibodies and assess immune efficacy.
High Sensitivity and Wide Dynamic Range
Antigen Concentration Optimization Results
With 20 nM PD-L1 antigen, the mouse anti-PD-L1 antibody showed a linear response (S/N ≥ 3) within the range of 100 ng/mL -- 80 μg/mL.
Strong Anti-Matrix Interference Ability
Testing in Different Matrix Solutions
In RPMI medium containing 10% FBS, the detection signal was highly consistent with that in the dedicated buffer, demonstrating its adaptability to complex samples.
Efficient Differentiation of Positive/Negative Clones
Hybridoma Clone Screening Results
The KeyTec® TR-FRET Hybridoma Screening Kit, rely on:
✅ Simplified three-step process: "Add-Incubate-Read";
✅ Ultra-fast 2-hour screening (supports overnight incubation for higher sensitivity);
✅ Covers His/FLAG/Fc/Biotinylated antigens and Mouse/Rat IgG;
✅ 384-well plate compatibility (20 μL system).
Has become the new gold standard replacing ELISA. Whether for early-stage hybridoma positive clone screening or antibody affinity ranking, it helps researchers:
Save Operation Time / Reduce Experimental Costs / Enhance Data Reliability
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