Protein-protein interaction is fundamental to signal transduction both within and between cells, as well as to metabolic regulation and biological reactions. Consequently, in-depth studies of these interactions not only enhance our understanding of intracellular biological mechanisms but also hold significant importance for drug research and development, as well as disease treatment. The KeyTec® TR-FRET Toolbox series offers a straightforward and rapid means of detecting protein-protein interactions, making it an excellent choice for drug screening and research into drug action mechanisms.
KeyTec® TR-FRET Protein A-LA is designed for developing the TR-FRET Assay. Protein A exhibits high affinity towards various species of Ig, particularly IgG. In the Antigen-Antibody Interaction assay, the IgG antibody binds to the acceptor (KeyTec® TR-FRET Protein A-LA*2), and the antigen is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Eu*1). When the Antigen-Antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of antigen-antibody interaction.
KeyTec® TR-FRET mAb anti-HIS-Green is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-HIS-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-GST-Green is designed for developing the TR-FRET Assay. The anti-GST antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one GST-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-GST-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
KeyTec® TR-FRET mAb anti-FLAG-Green is designed for developing the TR-FRET Assay. The anti-FLAG antibody is a rabbit monoclonal antibody. In the Protein-Protein Interaction assay, one FLAG-tagged protein binds to the acceptor (KeyTec® TR-FRET mAb anti-FLAG-Green*1), and the other protein is labeled (directly or indirectly) with the donor (KeyTec® TR-FRET Tb*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 520 nm, proportional to the extent of protein interaction.
This Green2-labeled anti-HA tag antibody serves as an acceptor reagent for TR-FRET-based protein-protein interaction assays. When paired with Solar Tb1-labeled anti-His tag antibody, it enables detection of interactions between His-tagged and HA-tagged proteins. The proximity-dependent FRET signal, read at 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.
This Green2-labeled Streptavidin serves as an acceptor reagent for TR-FRET-based protein-protein interaction assays. When paired with Solar Tb1-labeled anti-His tag antibody, it enables detection of interactions between His-tagged proteins and biotinylated binding partners. The proximity-dependent FRET signal, read at 520 nm, correlates with binding strength, providing a homogeneous and high-throughput platform for screening protein-protein interaction modulators.
KeyTec® TR-FRET Bright Eu is designed for developing the TR-FRET Assay. The anti-HIS antibody is a mouse monoclonal antibody. In the Protein-Protein Interaction assay, one HIS-tagged protein binds to the Donor (KeyTec® TR-FRET mAb anti-HIS-Bright Eu*1), and the other protein is labeled (directly or indirectly) with the Acceptor (KeyTec® TR-FRET LA/HX*2). When the two proteins interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.
This TR-FRET-based immunoassay uses Eu1-labeled anti-GST and LA2-labeled anti-His antibodies to detect proximity between GST- and His-tagged interacting proteins. Upon donor excitation, FRET produces a 665‑nm signal proportional to binding strength, enabling robust screening of PPI modulators such as small-molecule inhibitors or blocking antibodies.
This TR-FRET-based assay employs an Eu1-labeled anti-FLAG antibody and an LA2-labeled anti-His antibody to monitor the proximity of FLAG- and His-tagged interacting proteins. Upon donor excitation, FRET generates a 665‑nm emission signal that correlates directly with binding strength, providing a reliable platform for high-throughput screening of PPI modulators, including small-molecule inhibitors and neutralizing antibodies.
This Bright Tb1-labeled Streptavidin serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled anti-His tag antibody, it enables detection of interactions between biotinylated proteins and His-tagged partners. The proximity-dependent FRET signal, readable at both 665 nm and 520 nm, correlates with binding strength, offering a versatile and high-throughput platform for screening protein-protein interaction modulators.
This Bright Tb1-labeled anti-His tag antibody serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled Streptavidin, it enables detection of interactions between His-tagged proteins and biotinylated binding partners. The proximity-dependent FRET signal, measurable at both 665 nm and 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.
This Bright Tb1-labeled anti-GST tag antibody serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled anti-His tag antibody, it enables detection of interactions between GST-tagged and His-tagged proteins. The proximity-dependent FRET signal, measurable at both 665 nm and 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.
This Bright Tb1-labeled anti-FLAG tag antibody serves as a donor reagent for TR-FRET-based protein-protein interaction assays. When paired with Acceptor2-labeled anti-His tag antibody, it enables detection of interactions between FLAG-tagged and His-tagged proteins. The proximity-dependent FRET signal, measurable at both 665 nm and 520 nm, correlates with binding strength, providing a sensitive and homogeneous platform for high-throughput screening of protein-protein interaction modulators.
This TR-FRET-based assay utilizes a Tb1-labeled anti-HA antibody and an Acceptor2-labeled anti-His antibody to detect proximity between HA- and His-tagged interacting proteins. Upon donor excitation, FRET produces emission signals at both 665 nm and 520 nm, with signal intensities reflecting the binding strength. This dual-wavelength readout offers a robust and flexible platform for high-throughput screening of PPI modulators, including small-molecule inhibitors and blocking antibodies.
The KeyTec® TR - FRET Tool - Box series of products provide flexible solutions for developing protein - protein interaction experiments. This series includes tag antibodies (such as Anti - HIS, Anti - GST, etc.), secondary antibodies (such as Anti - Human IgG, etc.) and proteins (Streptavidin, Protein A, etc.) conjugated with TR-FRET fluorescence resonance energy transfer donors (Solar Eu/Solar Tb) or fluorescence resonance energy transfer acceptors (LA/HX), allowing you to develop your own TR-FRET experiments like 'building blocks'.
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